an agar well diffusion bioassay method for determination of clarithromycin in human plasma, using micrococcus luteus atcc 9341 as the assay organism, was compared with a selective high-performance liquid chromatographic (hplc) method with uv detection. spiked plasma was used to prepare standard and control samples for both methods. the results of the bioassay analyses with spiked plasma samples...

Although vitamin B12 can be assayed biologically with mice, chicks or rats, the microbiological method of assay is preferred since it is more rapid and economical. One serious shortcoming of many microbiological B12 assay procedures is the lack of specificity when crude materials are assayed. Crude materials can contain inhibitory or stimulatory factors which cause serious errors in assay resul...

The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in...

PDF.The online version of Theory and Application medicare application 2008 pdf of Microbiological Assay by. CHAPTER 10 DIFFUSION ASSAY METHODS FOR VITAMINS.for Microbiological. These media contain all the necessary nutriments for the growth of specified test organisms for the. B P Kelleher, S D 0 Broin. A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plate...

Chartreusin was produced in the fermentation liquors of Streptomyces chartreusis at peak concentrations of 200 to 300 mug/ml. The titers could be increased by 200 to 300% or more by incorporating d-fucose, a part of the chartreusin molecule, into the fermentation media. A microbiological assay with Sarcina lutea could detect concentrations of the drug of 0.5 to 1.0 mug/ml.

A newly developed radioimmunoassay was used to measure the concentration of vancomycin in 137 specimens of serum from patients being treated with this antibiotic. Of these sera, 84 were also analyzed with a microbiological assay technique for vancomycin. Duplicate determinations were done with each of the techniques. Individual values and averaged values for both methods were used for statistic...